The altered expression of cytoskeletal and synaptic remodeling proteins during epilepsy
Author:
Xin Yanbao1, Lin Guojiao1, Hua Tianbao2, Liang Jianmin34, Sun Tianmeng12, Wu Xuemei34
Affiliation:
1. Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, Institute of Immunology, The First Hospital of Jilin University , Changchun 130021 , China 2. The First Hospital and International Center of Future Science, Jilin University , Changchun 130015 , China 3. Department of Pediatric Neurology, The First Hospital of Jilin University , 1 Xinmin Street , Changchun 130000 , Jilin Province , China 4. Jilin Provincial Key Laboratory of Pediatric Neurology , Changchun 130021 , China
Abstract
Abstract
The cytoskeleton plays an important role in epilepsy; however, the mechanism is unknown. Therefore, this study aimed to reveal the mechanism of cytoskeletal proteins in epilepsy by investigating the expression of cytoskeletal proteins and synaptophysin (SYP) in mice at 0, 3, 6, and 24 h, 3 days, and 7 days in a kainic acid (KA)-induced epileptic model. Our results demonstrated that the expression of F-actin decreased significantly between 3 and 6 h, 6 and 24 h, and 24 h and 3 days (P < 0.05). Meanwhile, the expression of the neurofilament light chain, neurofilament medium chain, and neurofilament heavy chain subunits was significantly decreased (P < 0.001) at 3 h after the KA injection compared to the KA 0 h group, followed by an elevation at 6 h and a further decrease at 24 h compared to at 6 h. SYP expression was significantly decreased between 0 and 3 h as well as between 3 and 6 h (P < 0.05). At 24 h, the level was increased compared to at 6 h and continued to increase at 3 days after the KA injection. Thus, we propose that cytoskeletal proteins may be involved in the pathogenesis of epilepsy.
Publisher
Walter de Gruyter GmbH
Subject
General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience
Reference35 articles.
1. Feigin VL, Stark BA, Johnson CO, Roth GA, Bisignano C, Abady GG, et al. Global, regional, and national burden of stroke and its risk factors, 1990-2019: a systematic analysis for the Global Burden of Disease Study 2019. Lancet Neurol. 2021;20(10):795–820. 10.1016/s1474-4422(21)00252-0. 2. Beghi E, Giussani G, Nichols E, Abd-Allah F, Abdela J, Abdelalim A, et al. Global, regional, and national burden of epilepsy, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet Neurol. 2019;18(4):357–75. 10.1016/s1474-4422(18)30454-x. 3. Ding D, Zhou D, Sander JW, Wang W, Li S, Hong Z. Epilepsy in China: major progress in the past two decades. Lancet Neurol. 2021;20(4):316–26. 10.1016/s1474-4422(21)00023-5. 4. Neubauer BA. Epilepsy in neuropediatrics. Neuropediatrics. 2021;52(2):71–2. 10.1055/s-0041-1726339. 5. Kakooza-Mwesige A, Bakare M, Gaddour N, Juneja M. The need to improve autism services in lower-resource settings. Lancet. 2022;399(10321):217–20. 10.1016/s0140-6736(21)02658-1.
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