Neuroanatomy of melanocortin-4 receptor pathway in the mouse brain

Author:

Wang Kun1,Mao Wei2,Zhang Xiaoyu3,Zhao Yufei24,Fan Kuikui24,Pan Deng24,Liu Haodong24,Li Penghui24,Hai Rihan3,Du Chenguang234

Affiliation:

1. Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050000, China

2. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China

3. Vocational and Technical College, Inner Mongolia Agricultural University, Baotou 014109, China

4. Inner Mongolia Key Laboratory of Basic Veterinary Science, Hohhot 010018, China

Abstract

AbstractObjectiveMelanocortin-4 receptors (MC4Rs) are key regulators of energy homeostasis and adipose deposition in the central nervous system. Considering that MC4R expression regions and function-related research mainly focus on the paraventricular nucleus (PVN), little is known about their distribution throughout the mouse brain, although its messenger RNA distribution has been analyzed in the rat. Therefore, MC4R protein localization in mouse neurons was the focus of this study.MethodsMC4R protein distribution was assessed in mice through immunofluorescence and Western blotting.ResultsMC4R was differentially expressed throughout the arcuate nucleus (ARC), nucleus of the solitary tract (NTS), raphe pallidus (RPa), medial cerebellar nucleus, intermediolateral nucleus, and brainstem. The highest MC4R protein levels were found in the ARC and ventromedial hypothalamic nucleus, while they were significantly lower in the parabrachial nucleus and NTS. The lowest MC4R protein levels were found in the PVN; there was no difference in the protein levels between the area postrema and RPa.ConclusionsThese data provide a basic characterization of MC4R-expressing neurons and protein distribution in the mouse brain and may aid further research on its role in energy homeostasis.

Publisher

Walter de Gruyter GmbH

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

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