Affiliation:
1. Department of Stomatology, The Lianyungang Affiliated Hospital of Xuzhou Medical University , #182 Tongguan Road , Lianyungang 222002, Jiangsu Province , China
2. Department of Anesthesia, The Maternal and Child Health Hospital of Lianyungang City , Lianyungang 222006, Jiangsu Province , China
Abstract
Abstract
This study aimed to investigate the effect of periostin (PN) on the expression of receptor activator of nuclear factor-κB ligand (RANKL), osteoprotegerin (OPG), microtubule-associated protein1 light chain 3B (LC3B), and Beclin1 in mouse alveolar bone specimens and cultured osteoblasts in vitro, to preliminarily explore the role of PN and autophagy in remodeling bone metabolism during tooth eruption. Mice at 5 days of age were injected with 75 ng/mL recombinant PN protein under the periosteum for 3 consecutive days according to the standard of 1 mL/100 g/day. Then, their mandibles were removed, and the expression of bone metabolic and autophagy factors was detected by immunohistochemistry. Mouse osteoblast-like cells cultured in vitro were treated with recombinant PN at a concentration of 75 ng/mL. The changes in the aforementioned indicators were compared again by immunofluorescence and western blotting 72 h after dosing. The results of the mouse samples showed that the protein expression of RANKL, LC3B, and Beclin1 decreased, accompanied by the decrease in RANKL/OPG ratio. However, OPG protein expression increased in the dosing group. Immunofluorescence and western blotting results of osteoblasts cultured in vitro showed that the protein expression of RANKL, LC3B, Beclin1, and the RANKL/OPG ratio in the experimental group decreased, but OPG expression increased. PN may regulate alveolar bone metabolism during tooth eruption by inhibiting the RANKL/OPG ratio and autophagy, which will provide a new research perspective for further exploration of the mechanisms during tooth eruption.
Subject
General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience
Reference33 articles.
1. Richman JM. Shedding new light on the mysteries of tooth eruption. Proc Natl Acad Sci USA. 2019;116(2):353–5.
2. Nakao Y, Hikiji H, Okinaga T, Takeuchi J, Habu M, Yoshiga D, et al. Accumulation of hyaluronic acid in stromal cells modulates osteoclast formation by regulation of receptor activator of nuclear factor kappa-B ligand expression. Biochem Biophys Res Commun. 2019;512(3):537–43.
3. Wang SY, Xu SC, Shi ZW, Wu JC, Lei SH, Wang YL. Progress of research on the relationship between calcitonin gene-related peptide and RANK/RANKL/OPG system in the bone reconstruction. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2019;33(4):511–5.
4. Nishida D, Arai A, Zhao LJ, Yang MY, Nakamichi Y, Horibe K, et al. RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp. Sci Rep. 2021;11(1):4575.
5. Wei W, An YL, An Y, Fei DD, Wang QT. Activation of autophagy in periodontal ligament mesenchymal stem cells promotes angiogenesis in periodontitis. J Periodontol. 2018;89(6):718–27.