Phylogenetic analysis of promoter regions of human Dolichol kinase (DOLK) and orthologous genes using bioinformatics tools

Author:

Farooqi Nadia1,Rahman Ataur2,Ali Yasir3,Ali Kishwar4,Khan Muhammad Ezaz Hasan4,Jones David Aaron5,Abdelkarim Mouadh4,Ullah Farman6,Jalil Fazal3

Affiliation:

1. Department of Zoology, Women Campus, University of Swat , 19120 , Swat , Pakistan

2. Department of Botany, Laboratory of Plant Ecology and Dendrochronology, University of Malakand , Khyber Pakhtunkhwa , Pakistan

3. Department of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan , 23200 , Mardan , Pakistan

4. College of General Education, University of Doha for Science and Technology , Al Tarafa, Jelaiah Street, Duhail North, PO Box 24449 Doha , Qatar

5. College of Health Sciences, University of Doha for Science and Technology , Al Tarafa, Jelaiah Street, Duhail North, PO Box 24449 Doha , Qatar

6. Centre for Biotechnology and Microbiology, University of Swat , 19120 , Swat , Pakistan

Abstract

Abstract The Dolichol kinase (DOLK) gene encodes the polytopic DOLK protein associated with the endoplasmic reticulum (ER) N-glycosylation pathway catalyzing the final step in the biosynthesis of dolichol phosphate. Dolichol phosphate is an oligosaccharide carrier required for N-glycosylation of DOLK protein, with its deficiency leading to a severe hypo glycosylation phenotype in humans which can cause congenital disorders of glycosylation and death in early infancy. The aim of the present study is to identify the phylogenetic relationship between human and ortholog species based on their conserved sequences in DOLK gene. Sequence alignment of DOLK was carried out in this study and the evolutionarily conserved regulatory sequences were identified using bioinformatics. Promoter sequence of human DOLK was compared with orthologous sequences from different organisms. Conserved non-coding sequences (CNS) and motifs in promoter regions were found by analyzing upstream promoter sequences of Homo sapiens DOLK and its orthologous genes in other organisms. Conserved sequences were predicted in the promoter regions in CNS1 and CNS2. Conserved protein sequences were also identified by alignment of the orthologous sequences. Organisms with similar gene sequences are assumed to be closely related and the ER N-glycosylation pathway is conserved in them.

Publisher

Walter de Gruyter GmbH

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

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