Profiling of proteins and proteases in the products of the salivary gland, digestive tract and excretions from larvae of the camel nasal botfly, Cephalopina titillator (Clark)

Author:

Yousef Hesham A.1,Afify Amira1,Meguid Afaf Abdel1,Hassan Hany M.2

Affiliation:

1. Faculty of Science, Entomology Department, Cairo University, Egypt

2. Immunology Department, Animal Reproduction Research Institute, Egypt

Abstract

Abstract Proteins and proteolytic activities in the contents of the salivary gland (SGc), digestive tract (DTc) and excretory-secretory products (ESP) from larvae of the camel nasal botfly Cephalopina titillator were separated electrophoretically, and characterized. The protein profiles of the different samples were qualitatively quite similar in the larval stages L2 and L3. Zymogram analysis of proteases in the samples indicated that the digestive tract contained a greater variety of proteases than the salivary gland or the excretory-secretory products. They are mainly serine proteases. Proteases of ESP and DTc (especially of 3rd instar) contain trypsin- and chymotrypsin-like serine proteases, while the serine proteases of SGc are not of the trypsin- or chemotrypsin-type.

Publisher

Walter de Gruyter GmbH

Subject

General Biochemistry, Genetics and Molecular Biology

Reference33 articles.

1. Zumpt F. Myiasis in man and animal in the old world. Butterworth, London, 1965:267.

2. Zayed AA, Abdel-Meguid A, Madbouly MH, El-Moursy AA, El-Khateeb RM. Incidence and Monthly prevalence of Cephalopin titillator (Diptera: Oestridae) larvae infesting dromedary camels slaughtered at Egyptian abattoir. J Egypt Vet Med Assoc 1994;54:13–22.

3. Otranto D. The immunology of myiasis: Parasite survival and host defense strategies. Trends in Parasitol 2001;17:176–82.

4. Oryan A, Valinezhad A, Moraveji M. Prevalence and pathology of camel nasal myiasis in eastern areas of Iran. Trop Biomed 2008;25:30–6.

5. Shakerian A, Hosseini SR, Abbasi M. Prevalence of Cephalopina titillator (Diptera: Oestridae) larvae in one-humped camel (Camelus dromedaries) in Najaf-abad, Iran. Global Veterinaria 2011;6:320–3.

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