Affiliation:
1. Radiobiology Department , National Veterinary Research Institute , 24-100 Pulawy , Poland
Abstract
Abstract
Introduction: Polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) belong to a well-known group of pollutants. Present in feedstuffs, they bioaccumulate in tissues of food-producing animals. Food is the source of over 90% of human PCDD/Fs and DL-PCBs intake. Thus, feed control is one of the pillars of the EU strategy and a mean of reducing human exposure. The article presents AhR based reporter gene bioassay method for PCDD/Fs and DL-PCBs analysis in feed and its validation results.
Material and Methods: Analytes were extracted from samples with fat. Subsequently, fat and other interferences were removed from extract using sulphuric acid modified silica. Extract was further cleaned and PCDD/Fs separated from DL-PCBs using carbon column. Contaminants detection was performed using H1L6.1c3 cell line, which produces luciferase in response to AhR ligands present in extract.
Results: Performance characteristics (repeatability, reproducibility, and apparent recovery) fulfil the requirements of Regulation 2017/771/EU. The positive correlation between bioassay and reference HRGC-HRMS method was confirmed. Moreover, the role of screening method used in connection with the confirmatory HRGC-HRMS method in providing feed and food safety has been discussed.
Conclusion: Bioassay is a useful method for dioxin and DL-PCBs analysis, allowing cost reduction of monitoring programmes with minimal risk of false negative results.
Reference29 articles.
1. 1. Behnisch P.A., Allen R., Anderson J., Brouwer A., Brown D.J., Campbell T.C., Goeyens L., Harrison R., Hoogenboom R., van Overmeire I., Traag W., Malisch R.: Harmonised quality criteria for chemical and bioassays analyses of PCDDs/PCDFs in feed and food. Part 2: general considerations, bioassay methods. Organohalogen Comp 2001, 50, 59–63.
2. 2. Bernard A., Broeckaert F., De Poorter G., De Cock A., Hermans C., Saegerman C., Houins G.: The Belgian PCB/dioxin incident: analysis of the food chain contamination and health risk evaluation. Environ Res 2002, 88, 1–18.10.1006/enrs.2001.4274
3. 3. Besselink H., Brouwer B., van der Burg B.: Validation and regulatory acceptance of bio-based approaches to assure feedstock, water product quality in a bio-based economy. Ind Crops Prod 2017, 106, 138–145.
4. 4. Birnbaum L.S.: The mechanism of dioxin toxicity: relationship to risk assessment. Environ Health Perspect 1994, 102, 157–167.
5. 5. Denison M.S., Zhao B., Baston D.S., Clark G.C., Murata H., Han D.: Recombinant cell bioassay systems for the detection and relative quantitation of halogenated dioxins and related chemicals. Talanta 2004, 63, 1123–1133.10.1016/j.talanta.2004.05.032
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献