Expression of eIF6 and its relationship with cell proliferation in colorectal adenocarcinoma
Author:
Chen Liming1, Pang Jiuling2, Dong Huajiang3, Qu Bo4, Yang Tao5, Ma Xi1, Liu Qi2, Liu Aidong6ORCID
Affiliation:
1. Department of Anorectal Surgery , Tianjin Hospital, Tianjin University , Tianjin , China 2. Department of Anorectal Surgery , Tangshan Workers Hospital , Tangshan , China 3. Department of Health Services , Logistics University of the Chinese People’s Armed Police Force , Tianjin , China 4. Institute of Disaster and Emergency Medicine, Tianjin University , Tianjin , China 5. Department of General Surgery , Tianjin First Central Hospital , Tianjin , China 6. Department of Pathology , Tianjin Hospital, Tianjin University , Tianjin , China
Abstract
Abstract
Objectives
Eukaryotic translation initiation factors (eIFs) are genes related to tumor formation. They selectively translate some mRNAs, regulate protein synthesis, promote cell proliferation, and effectively control the progression of some tumors. As a member of this family, eukaryotic translation initiation factor 6 (eIF6) plays a crucial role in tumor formation and progression. Exploring the expression characteristics of eIF6 and proliferating cell nuclear antigen (PCNA), a proliferation-associated factor, in colorectal adenocarcinoma (CRAC) and analyzing their correlation and clinical significance can provide a theoretical basis for the study of CRAC and objective biological indicators for the clinical judgment of tumor formation and progression.
Methods
The GEPIA database was used to predict the trend of eIF6 and PCNA in CRAC. Sixty-four patients diagnosed with CRAC and undergoing surgical treatment were selected from January 2017 to November 2022. CRAC tissues and normal mucous tissues (>3 cm away from the tumor margin) were retained. The expression of eIF6 and PCNA was detected by immunohistochemistry (IHC). Colon cancer cell lines SW480, HCT15, and SW620 and normal colon cell line NCM460 were selected, and the expression of eFI6 and PCNA in these cells was detected by Western blot.
Results
A trend of elevated expression of eIF6 and PCNA was predicted in CRAC by the GEPIA database. The expression levels of eIF6 (65.63 % vs. 9.38 %, X2=43.2000, p<0.0001) and PCNA (84.38 % vs. 46.88 %, X2=19.9481, p<0.0001) were higher in CRAC tissues than that in normal mucosa as indicated by IHC. Significant difference in eIF6 and PCNA expression was found among different maxim tumor diameters and depths of infiltration in colon adenocarcinoma (p<0.05). No statistical difference in eIF6 and PCNA expression was observed among different genders, ages, degrees of differentiation, LNMets, intravascular cancer thrombosis, TNM stages, and numbers of lymph node metastases (p>0.05). A positive correlation was found between eIF6 and PCNA in CRAC (X2=5.05, r=0.77, p=0.0283). Their expression was significantly higher in SW480, HCT15, and SW620 than in NCM460 (p<0.05).
Conclusions
eIF6 is highly expressed in CRAC, participates in tumor formation and progression, and has a positive correlation with PCNA.
Publisher
Walter de Gruyter GmbH
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