Effects of bacterial populations, temperature and exogenous hydrogen peroxide on the induction of the hypersensitive response in Nicotiana tabacum against Xanthomonas perforans

Abstract

AbstractThe objective of this study was to investigate the effects of inoculum concentration, plant post-inoculation incubation temperature and exogenous hydrogen peroxide (H2O2) on the induction of the hypersensitive response (HR) inNicotiana tabacumagainstXanthomonas perforans. Inoculation of leaves withX. perforansat a concentration of 108CFU · ml−1and incubation of plants at 30°C resulted in the strongest HR elicitation. Furthermore, an exogenous supply of H2O2acceleratedX. perforans-induced HR, whereasin plantaH2O2removal by application of catalase led to a delay in HR development. Our data suggest that H2O2has an important role in HR ofN. tabacumagainstX. perforans.