Author:
Mokrini Fouad,Viaene Nicole,Waeyenberge Lieven,Dababat Abdelfattah A.,Moens Maurice
Abstract
AbstractMorphological and molecular diversity among 11 populations of cereal cyst nematodes from different wheat production areas in Morocco was investigated using light microscopy, species-specific primers, complemented by the ITS-rDNA sequences. Morphometrics of cysts and second-stage juveniles (J2s) were generally within the expected ranges forHeterodera avenae; only the isolate from Aïn Jmaa showed morphometrics conforming to those ofH. latipons. When using species-specific primers forH. avenaeandH. latipons, the specific bands of 109 bp and 204 bp, respectively, confirmed the morphological identification. In addition, the internal transcribed spacer (ITS) regions were sequenced to study the diversity of the 11 populations. These sequences were compared with those ofHeteroderaspecies available in the GenBank database (www.ncbi.nlm.nih.gov) and confirmed again the identity of the species. Ten sequences of the ITS-rDNA were similar (99–100%) to the sequences ofH. avenaepublished in GenBank and three sequences, corresponding with one population, were similar (97–99%) toH. latipons.
Subject
Plant Science,Soil Science,Agronomy and Crop Science
Reference32 articles.
1. Handling fixing staining mounting nematodes In Laboratory for with and Nematodes ed CAB International London UK;Hooper;Methods Work Plant Soil,1986
2. of a species - specific PCR to detect the cereal cyst nematode latipons;Toumi;Development Nematology,2013
3. Comparisons of isolates ofHeterodera avenaeusing protein patterns and ribosomal DNA of;Ferris;Journal Nematology,1994
4. Identification ofHeterodera avenaegroup species by morphometrics and rDNA;Subbotin;Nematology,1999
5. aucklandicasp from a New Zealand native grass with notes on the species of theH avenaegroup http dx doi org;Wouts;New Zealand Journal of Zoology,1995
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