Affiliation:
1. Institut für Biochemie der Universität Graz
2. Institut für M athematische Statistik der Technischen Universität Graz
Abstract
Abstract
The num ber of SH-groups of the soluble proteins (Prot-SH) of 12 different types of animal tum ors and of cultivated chicken fibroblasts increases with decreasing doubling tim e (td). Between Prot-SH and td an inverse association was found with a significance level of 99.5%.
After 30 min incubation in vitro, 4-hydroxypentenal (HPE) reacts with different am ounts of the Prot-SH of the investigated cells and tissues. It was found that the absolute, as well as the per cental amounts of HPE-reactive Prot-SH increase with decreasing td, each of the respective inverse associations was found to be highly significant. The tum ors were collected in three groups, each with a corresponding range of td and a mean value for Prot-SH . Taking the Prot-SH of the slowest growing tissue (DENA-hepatoma) as the reference value, the increments of Prot-SH were cal culated for each of the three groups and were found to be strikingly similar to the number of HPE-reactive Prot-SH. The single Prot-SH increm ents of the diverse cell and tissue types compared with DENA-hepatoma were found to be highly significantly and inversely associated with the respective Prot-SH decreases caused by HPE.
Hence it may be concluded that HPE reacts preferably with those of the Prot-SH which are of some functional importance for tum or growth and, moreover that the attack on those thiols is the more effective the faster the tumor is growing.
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
8 articles.
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