Handling of lipemic samples in the clinical laboratory-Reference-Cited by-同舟云学术

Handling of lipemic samples in the clinical laboratory

Published:2023-02-20 Issue:1 Volume:4 Page:5-15
ISSN:2628-491X
Container-title:Advances in Laboratory Medicine / Avances en Medicina de Laboratorio
language:en
Short-container-title:

Author:

Fernández Prendes Carla¹²,Castro Castro María José²³,Sánchez Navarro Lourdes⁴,Rapún Mas Loreto⁴,Morales Indiano Cristian¹²,Arrobas Velilla Teresa⁵²

Affiliation:

1. Laboratory Medicine Department, Laboratori Clínic Metropolitana Nord , Hospital Universitari Germans Trias I Pujol , Badalona , Spain

2. Workgroup of Lipoproteins and Cardiovascular Diseases, Spanish Society of Laboratory Medicine , Barcelona , Spain

3. Biochemistry Core, Laboratori Clínic Territorial Metropolitana Sud , Hospital Universitari de Bellvitge , L’Hospitalet de Llobregat , Spain

4. Haematological Core, Laboratori Clínic Territorial Metropolitana Sud , Hospital Universitari de Bellvitge , L’Hospitalet de Llobregat , Spain

5. Laboratory of Nutrition and Cardiovascular Risk , Hospital Universitario Virgen Macarena , Sevilla , Spain

Abstract

Abstract Interferences in the clinical laboratory may lead physicians misinterpret results for some biological analytes. The most common analytical interferences in the clinical laboratory include hemolysis, icterus and lipemia. Lipemia is defined as turbidity in a sample caused by the accumulation of lipoproteins, mainly very-low density lipoproteins (VLDL) and chylomicrons. Several methods are available for the detection of lipemic samples, including the lipemic index, or triglyceride quantification in serum or plasma samples, or mean corpuscular hemoglobin (MCHC) concentration in blood samples. According to the European Directive 98/79/CE, it is the responsibility of clinical laboratories to monitor the presence of interfering substances that may affect the measurement of an analyte. There is an urgent need to standardize interference studies and the way interferences are reported by manufacturers. Several methods are currently available to remove interference from lipemia and enable accurate measurement of biological quantities. The clinical laboratory should establish a protocol for the handling of lipemic samples according to the biological quantity to be tested.

Publisher

Walter de Gruyter GmbH

Subject

Medical Laboratory Technology,Education,Medicine (miscellaneous)

Link

https://www.degruyter.com/document/doi/10.1515/almed-2023-0003/pdf

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