Association of S19W polymorphism in APOA5 gene and serum lipid levels in patients with type 2 diabetic nephropathy

Abstract

Abstract Objectives Type 2 diabetic Mellitus (T2DM) is the most common systemic and endocrine disease in humans, and diabetic nephropathy is one of the most serious complications of this disorder. The polymorphisms in the apolipoprotein A5 (ApoA5) gene are strongly related to hypertriglyceridemia and are considered a predisposing factor for diabetic nephropathy. The current study proposed to examine the association of APOA5-S19W polymorphism with serum lipids levels in patients with type 2 diabetic nephropathy in Mazandaran province. Methods This case-control study was designed to determine the association of APOA5–S19W polymorphism with plasma lipid profile in 161 T2DM patients with nephropathy (DN+), without nephropathy (DN−), and in 58 healthy individuals. Lipid profile values were measured using Pars Azmoun commercial kits. S19W variant, one of the polymorphisms of the APOA5 gene, was determined by PCR–restriction fragment length polymorphism (PCR-RFLP) and Taq1 restriction enzyme. Results In comparison between the three groups, DN+ had a higher mean TG than DN− and the control group (p<0.001). The incidence of the G allele in DN+ was not significant compared to groups of DN−. Comparing the relationship between the mean of biochemical variables with CC and CG genotypes showed that the mean level of TG in people with CC genotype was increased compared to people with CG genotype in diabetic patients. However, this increase was not significant (p=0.19). Conclusions There was no association between SNP APOA5 S19W and serum lipids in diabetic patients with and without nephropathy.