Assessment of AXL and mTOR genes expression in medullary thyroid carcinoma (MTC) cell line in relation with over expression of miR-144 and miR-34a
Author:
Pishkari Shaghayegh1, Hadavi Razie2ORCID, Koochaki Ameneh3ORCID, Razaviyan Javad4ORCID, Paryan Mahdi5ORCID, Hashemi Mehrdad1ORCID, Mohammadi-Yeganeh Samira36ORCID
Affiliation:
1. Department of Medicine , Tehran Medical Sciences Branch, Islamic Azad University , Tehran , Iran 2. Department of Biochemistry, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran 3. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences , Tehran , Iran 4. Student Research Committee, Department of Biochemistry , School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran , Iran 5. Department of Research and Development , Production and Research Complex, Pasteur Institute of Iran , Tehran , Iran 6. Department of Medical Biotechnology, School of Advanced Technologies in Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran
Abstract
Abstract
Objectives
The aim of the present study was to investigate the expression of AXL and mTOR genes and their targeting microRNAs (miRNAs) including miR-34a and miR-144 in Medullary Thyroid Carcinoma (MTC) cell line, TT, and determine the effect of these two miRNAs on their target genes to introduce new molecular markers or therapeutics.
Methods
The expression of miR-34a, miR-144, and their targets genes including AXL and mTOR was evaluated by quantitative Real-time PCR. Luciferase assay was performed to confirm the interaction between miRNAs and their target mRNAs. The expression level of AXL and mTOR was evaluated before and after miRNAs induction in TT cell line compared with Cos7 as control cells.
Results
The expression of AXL and mTOR were up-regulated significantly, while miR-34a and miR-144 were down-regulated in TT cell line compared to Cos7. After transduction, the overexpression of miR-34a and 144 caused down-regulation of both genes. Luciferase assay results showed that the mTOR is targeted by miR-34a and miR-144 and the intensity of luciferase decreased in the presence of miRNAs.
Conclusions
Based on the results of the present study and since AXL and mTOR genes play a critical role in variety of human cancers, suppression of these genes by their targeting miRNAs, especially miR-34a and miR-144, can be propose as a new strategy for MTC management. However, more studies are needed to approve the hypothesis.
Publisher
Walter de Gruyter GmbH
Subject
Endocrinology,Molecular Biology,General Medicine,Endocrinology, Diabetes and Metabolism
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