Potential tumor marker for hepatocellular carcinoma identification: PI3K and pro-inflammatory cytokines (TGF-β, IL-1, and IL-6)
Author:
Tabakhiyan Fahimeh1, Mir Amirabbas2, Vahedian Vahid3
Affiliation:
1. Department of Biology, Ahar Branch , Islamic Azad University , Ahar , Iran 2. Institute of Nano Science and Nano Technology, University of Kashan , Kashan , Islamic Republic of Iran 3. Cancer Biology Research Group, Faculty of Medicine Institute of Biotechnology (FMB-IBTEC) Sao Paulo State University (UNESP) , Sao Paulo , Brazil
Abstract
Abstract
Objectives
Hepatocellular carcinoma (HCC), the most common form of liver cancer, is a leading cause of tumor-associated mortality worldwide. Diagnosis based upon non-invasive criteria is currently challenged by the need for molecular information that requires tissue or liquid biopsies. The progression of HCC is often associated with chronic inflammation, expression levels of inflammatory mediators, chemokine, and cytokines. In this study, we try to evaluate the PI3K and pro-inflammatory cytokines, TGF-β, IL-1, and IL-6 expression level in patients with liver cancer.
Materials and methods
The kupffer cells were isolated from patient’s specimens. Real-time PCR was applied to evaluate the expression level of PI3K in cell lines or tumors. The concentrations of TGF-β, IL-1, and IL-6 were measured by the quantitative ELISA kit.
Results
PI3K mRNA expression in cancer cells was increased markedly vs. normal cells. The ELISA results demonstrated over expression of TGF-β, IL-1, and IL-6 in patients and positive correlation between tumor size and stage.
Discussion
This study suggests that targeting the expression level of PI3K and pro-inflammatory chemokine and cytokines, TGF-β, IL-1, and IL-6, may be a potential diagnostic strategy in HCC patients.
Publisher
Walter de Gruyter GmbH
Subject
Endocrinology,Molecular Biology,General Medicine,Endocrinology, Diabetes and Metabolism
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