Effect of Artemisia absinthium ethanolic extract on oxidative stress markers and the TLR4, S100A4, Bax and Bcl-2 genes expression in the kidney of STZ-induced diabetic rats
Author:
Bagheri Fatemeh1, Amri Jamal12, Salehi Mehdi1, Karami Hadi3, Alimoradian Abbas4, Latifi Seied Amirhossein1
Affiliation:
1. Traditional and Complementary Medicine Research Center , Arak University of Medical Sciences , Arak , Islamic Republic of Iran 2. Member of Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences , Arak , Islamic Republic of Iran 3. Department of Molecular Medicine and Biotechnology, Faculty of Medicine , Arak University of Medical Sciences , Arak , Islamic Republic of Iran 4. Department of Pharmacology, School of Medicine , Arak University of Medical Sciences , Arak , Islamic Republic of Iran
Abstract
Abstract
Objectives
The present study was conducted to examine antidiabetic effects of Artemisia absinthium ethanolic extract [A. absinthium] and to investigate its effects on oxidative stress markers and the expression of TLR4, S100A4, Bax and Bcl-2 genes in the kidney of STZ-induced diabetic rats.
Methods
Thirty six rats (weight 200–250 g) were randomly divided into diabetes and control groups. Induction of diabetes was performed using STZ (55 mg/kg.bw). Biochemical parameters and oxidative stress markers (SOD and MDA) were measured using spectrophotometry after 60 days of treatment. The expression of TLR4, S100A4, Bax and Bcl-2 were analyzed by real-time PCR. One-way analysis of variance (ANOVA) and Bonferroni post hoc test were used to compare the data.
Results
Diabetes significantly impairs the serum fasting blood glucose (FBG), lipid profile, urea, creatinine and albumin. At the end of treatment with A. absinthium extract, these parameters were close to the normal range. The results showed that the A. absinthium extract significantly decreased the kidney expression of TLR4, S100A4, Bax and increased the expression of Bcl-2 and improved oxidative stress markers (SOD and MDA) in the kidney tissues of treated rats. Also, all of these beneficial effects of the A. absinthium were dose-dependent.
Conclusions
The extract of A. absinthium possesses antidiabetic effects. A. absinthium decreased the expression of TLR4, S100A4, Bax and increased the expression of Bcl-2 and improved oxidative stress. Therefore, this herbal extract can be used as an adjuvant treatment for diabetic complications.
Publisher
Walter de Gruyter GmbH
Subject
Endocrinology,Molecular Biology,General Medicine,Endocrinology, Diabetes and Metabolism
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