Biofabrication of silver nanoparticles using diallyl sulphide (DAS) and comparison of cytotoxic potential of DAS-AgNPs and Kodaikanal Hill garlic in human cervical cancer
Author:
Priya Pranjal1, Maheswari Nallal Uma1, Razia Muthuswamy1, Sivaramakrishnan Sivaperumal2, Prabha Kannan3, Priya Lakshminarayanan Srimathi4, Kabeen Suresh Kumar56, Durga Karuppaiya56, Ayyar Manikandan56
Affiliation:
1. Department of Biotechnology , Mother Teresa Women’s University , Kodaikanal 624101 , Tamil Nadu , India 2. Department of Biotechnology , Bharathidasan University , Tiruchirappalli 620024 , Tamil Nadu , India 3. Department of Physics , Mother Teresa Women’s University , Kodaikanal 624101 , Tamil Nadu , India 4. Department of Natural Resource Management, Horticultural College and Research Institute (HC & RI) , Tamil Nadu Agricultural University (TNAU) , Periyakulam , Theni 625604 , India 5. Department of Chemistry , Karpagam Academy of Higher Education , Coimbatore 641021 , Tamil Nadu , India 6. Centre for Material Chemistry , Karpagam Academy of Higher Education , Coimbatore 641021 , Tamil Nadu , India
Abstract
Abstract
Garlic (Allium sativum) exhibit well defined therapeutic properties due to presence of distinct bioactive compounds, specifically organosulphur compounds. Spectral analysis by UV–Vis and FT-IR confirmed interaction of different functional groups and free electrons with the electromagnetic waves showed presence of different bioactive compounds formulated silver nanoparticles (AgNPs) using diallyl sulphide (DAS) also done for aqueous garlic extract. Different assays performed for confirming the presence of antibacterial and anticancer properties of DAS-AgNPs and garlic extract. The minimum inhibitory concentration required to inhibit Gram negative (Escherichia coli, V. cholera) and Gram positive (S. aureus, B. cereus) were found lesser in DAS-AgNPs followed by garlic extract. Cytotoxicity effect confirmed by DAS-AgNPs and A. sativum towards HeLa cell line by MTT assay. The IC50 values obtained for MTT assay were at 24.09 μg/ml in DAS-AgNPs and 129.22 μg/ml in garlic extract. Anticancer efficacy was found more potent in DAS-AgNPs as compared with aqueous garlic extract.
Publisher
Walter de Gruyter GmbH
Subject
Physical and Theoretical Chemistry
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