Optical and topographic characteristics of silver films deposited from a colloidal solution on polyelectrolytes for IgG-FITC fluorescence analysis
Author:
Kulakovich Olga1, Scherbovich Anastasia12, Koktysh Irina2, Melnikova Yanina2, Ramanenka Andrei1, Gaponenko Sergey1, Maskevich Sergey2
Affiliation:
1. B.I. Stepanov Institute of Physics, National Academy of Sciences of Belarus , 68-2 Nezaliezhnasci Avenue , 220072 , Minsk , Republic of Belarus 2. Belarusian State University, International Sakharov Environmental Institute of BSU , Dolgobrodskaya Str. , Minsk 220070 , Republic of Belarus
Abstract
Abstract
The optimization of the fluorescence enhancement factor of the IgG-FITC conjugate as an immunofluorescent marker, depending on the optical and topographic parameters of the colloidal silver film on the surface of a polystyrene plate for immunoassay, was carried out for the first time, and the factors effecting the enhancement were identified. By means of time-resolved spectroscopy as well as by detection the relative concentrations of IgG-FITC adsorbed on the solid phase with enzyme-linked immunoassay, it was shown that fluorescence enhancement in the presence of silver nanoparticles is a resonance process associated with plasmon effects. The most important parameters correlating with the fluorescence enhancement factor are the optical density value at the wavelength of excitation and emission of the fluorophore. The maximum enhancement factor of 10.2 times was obtained for Ag films with the highest optical density.
Funder
International Sakharov Environmental Institute of Belarusian State University with the Republican Foundation for Basic Research
Publisher
Walter de Gruyter GmbH
Subject
Physical and Theoretical Chemistry
Reference39 articles.
1. Lakowicz, J. R., Ray, K., Chowdhury, M., Szmacinski, H., Fu, Y., Zhang, J., Nowaczyk, K. Analyst 2008, 133, 1308; https://doi.org/10.1039/b802918k. 2. Li, M., Cushing, S. K., Wu, N. Analyst 2015, 140, 386; https://doi.org/10.1039/c4an01079e. 3. Kulakovich, O. S., Strekal, N. D., Artemyev, M. V., Stupak, A. P., Maskevich, S. A., Gaponenko, S. V. J. Appl. Spectrosc. 2006, 73, 892. 4. Gaponenko, S. V. Phys. Rev. B 2002, 65, 140303; https://doi.org/10.1103/physrevb.65.140303. 5. Attridge, J. W., Daniels, P. B., Deacon, J. K., Robinson, G. A., Davidson, G. P. Biosens. Bioelectron. 1991, 6, 201; https://doi.org/10.1016/0956-5663(91)80005-i.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|