The association of methylene tetrahydrofolate reductase (MTHFR) A1298C gene polymorphism, homocysteine, vitamin B12, and folate with coronary artery disease (CAD) in the north of Iran
Author:
Amani Saeideh1, Mirzajani Ebrahim23ORCID, Kassaee Seyed Mehrdad1, Mahmoudi Minoo1, Mirbolouk Fardin4
Affiliation:
1. Department of Biology , Hamedan Branch, Islamic Azad University , Hamedan , Islamic Republic of Iran 2. Department of Biochemistry , School of Medicine, Guilan University of Medical Sciences , Rasht , Islamic Republic of Iran 3. Cellular and Molecular Research Center, School of Medicine, Guilan University of Medical Sciences , Rasht , Islamic Republic of Iran 4. Department of Cardiology , Cardiovascular Diseases Research Center, Heshmat Hospital, School of Medicine, Guilan University of Medical Sciences , Rasht , Islamic Republic of Iran
Abstract
Abstract
Background
We pursued to find out the possible association of Methylene tetrahydrofolate reductase (MTHFR) A1298C gene polymorphism, blood homocysteine, vitamin B12, and folate with Coronary artery disease (CAD) in the study population in Guilan, north of Iran.
Material and Methods
Ninety patients with CAD and 76 healthy controls were evaluated. MTHFR A1298C polymorphism and its genotype frequency, the plasma level of homocysteine, vitamin B12 and folate were evaluated by using ARMS-PCR, ELISA, and Chemiluminescence methods, respectively.
Results
The frequency of genotypes, A, AC and CC in CAD were 40, 35.6, 24.4%, respectively which was significantly different (p=0.016) from the control group that were 26.3, 57.9 and 15.8%, respectively. The serum level of vitamin B12 and folate in genotype A1298C were not statistically significant between two groups (p>0.05), however, the plasma homocysteine in patients with CAD was remarkably higher than the control group (p<0.001). Additionally, in CAD patients the plasma level of homocysteine in the AC genotype was significantly higher than the control subjects (p=0.005).
Conclusion
It is thus concluded that MTHFR A1298C gene polymorphism is associated with CAD. It seems that the AC genotype of MTHFR A1298C polymorphism might have a protective effect on CAD.
Publisher
Walter de Gruyter GmbH
Subject
Biochemistry, medical,Clinical Biochemistry,Molecular Biology,Biochemistry
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