Effect of transportation and freeze-thaw procedure on hemostatic tests

Author:

Öngen İpek Belkız12ORCID,Sitar Mustafa Erinç3

Affiliation:

1. Maltepe University Faculty of Medicine , Research and Training Hospital, Central Medical Biochemistry Laboratory , Feyzullah Street No: 39 34845 Maltepe , Istanbul , Turkey

2. Maltepe University Faculty of Medicine , Department of Medical Biochemistry , Istanbul , Turkey , Phone: 00 90 444 0 620 – 2041, Fax: 00 90 216 399 00 60

3. Maltepe University Faculty of Medicine , Department of Medical Biochemistry , Istanbul , Turkey

Abstract

Abstract Objective Coagulation tests are sensitive to pre-analytical variables. The aim of our study is to identify the effect of transportation and freeze-thaw status on for Factor VIII, Factor IX, Anti-thrombin III, Protein S, Protein C, Prothrombin time (PT) and Activated partial thromboplastin time (aPTT). Materials and methods The study was performed on 102 plasma samples obtained from 34 healthy volunteers. The samples were divided into three groups. Group A was analyzed whereas group B, C were frozen at −20°C. After 24 h, group B and C were transported for 2 h. Following the transfer, group B was analyzed and C was frozen at −20°C. After 24 h, group C was analyzed. Analyses of samples were performed in Thrombolyzer-XRM for PT, aPTT, Factor VIII, Factor IX, Anti-thrombin III, Protein C and Protein S. Results There were significant variations for PT, aPTT, Protein S, Factor VIII and Factor IX for group A&B and A&C comparisons in different stability criteria approaches. In significant change limit and percentage change calculations Protein S, Factor VIII and IX showed significant differences. For acceptable change limit approach, aPTT and Factor IX showed significant changes. Conclusion Laboratories should take precautions for transportation and freeze-thaw cycles to prevent inaccurate results.

Publisher

Walter de Gruyter GmbH

Subject

Biochemistry (medical),Clinical Biochemistry,Molecular Biology,Biochemistry

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Turkish journal of biochemistry is an open access journal again;Turkish Journal of Biochemistry;2021-02-01

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