Topochemical Characterisation of Phenolic Extractives in Discoloured Beechwood (Fagus sylvatica L.)

Abstract

Summary The topochemical distribution of phenolic extractives in steamed and kiln-dried beechwood with discolourations was investigated on a cellular level by using scanning UV microspectrophotometry (UMSP). For the chemical characterisation of accessory compounds, acetone and methanol extracts of the discoloured beechwood were separated by accelerated solvent extraction (ASE) and analysed with high performance liquid chromatography (HPLC). The UV microscopic investigations reveal that the accessory compounds responsible for the discolouration of beechwood are mainly restricted to the longitudinal and ray parenchyma cells and the lumen of vessels. The detected extractives are characterised by high UV absorbance values and an absorbance maximum in a wavelength range between 280 and 290 nm. The separation of the acetone and methanol extracts of discoloured beechwood shows the presence of different low molecular phenols such as catechin and 2,6-dimethoxybenzochinon, which are transformed into high condensation compounds during steaming and kiln-drying.