Molecular Identity of Species and Isolates of Internal Pore Fungi Antrodia spp. and Oligoporus placenta

Abstract

Summary Antrodia vaillantii (DC.: Fr.) Ryv., A. serialis (Fr.) Donk, A. sinuosa (Fr.) P. Karsten, A. xantha (Fr.: Fr.) Ryv. and Oligoporus placenta (Fr.) Gilb.& Ryv. form a group of internal brown-rot fungi (‘Porenschwämme’), which are associated with decay of coniferous woodwork in buildings and timber in ground contact. The fungi have similar occurrence, biology, fruit bodies and mycelia. Their nomenclature has a confusing history and is still not uniform. For a better understanding of the domestic pore fungi and for a reliable species differentiation and identification of isolates, the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA) was amplified by polymerase chain reaction and sequenced. Isolates from decayed wood and from culture collections were used. The ITS sequences characteristic of A. vaillantii, A. serialis,A. sinuosa, A. xantha and O. placenta were obtained and deposited in the international databases. The ITS size ranges from 636 to 668 bp. Intraspecific variation was low. A dendrogram was performed for the phylogenetic relationship. Some isolates obtained mislabelled were named correctly according to the ITS sequence. The sequences contribute to our collection of ITS data from internal wood decay fungi made to characterize fungi in culture.