Phage-display reveals interaction of lipocalin allergen Can f 1 with a peptide resembling the antigen binding region of a human γδT-cell receptor-Reference-Cited by-同舟云学术

Phage-display reveals interaction of lipocalin allergen Can f 1 with a peptide resembling the antigen binding region of a human γδT-cell receptor

Published:2020-09-16 Issue:4 Volume:402 Page:433-437
ISSN:1437-4315
Container-title:Biological Chemistry
language:en
Short-container-title:

Author:

Habeler Matthias¹,Redl Bernhard¹^ORCID

Affiliation:

1. Institute of Molecular Biology , Medical University Innsbruck , Innrain 80 , A-6020 Innsbruck , Austria

Abstract

Abstract Although some progress has been achieved in understanding certain aspects of the allergenic mechanism of animal lipocalins, they still remain largely enigmatic. One possibility to unravel this property is to investigate their interaction with components of the immune system. Since these components are highly complex we intended to use a high-throughput technology for this purpose. Therefore, we used phage-display of a random peptide library for panning against the dog allergen Can f 1. By this method we identified a Can f 1 binding peptide corresponding to the antigen-binding site of a putative γδT-cell receptor. Additional biochemical investigations confirmed this interaction.

Funder

Austrian Science Fund

Publisher

Walter de Gruyter GmbH

Subject

Clinical Biochemistry,Molecular Biology,Biochemistry

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