Room temperature X-ray absorption spectroscopy of metalloenzymes with drop-on-demand sample delivery at XFELs-Reference-Cited by-同舟云学术

Room temperature X-ray absorption spectroscopy of metalloenzymes with drop-on-demand sample delivery at XFELs

Published:2023-04-19 Issue:8 Volume:95 Page:891-897
ISSN:0033-4545
Container-title:Pure and Applied Chemistry
language:en
Short-container-title:

Author:

Bogacz Isabel¹^ORCID,Makita Hiroki¹^ORCID,Simon Philipp S.¹^ORCID,Zhang Miao¹^ORCID,Doyle Margaret D.¹^ORCID,Chatterjee Ruchira¹^ORCID,Fransson Thomas²^ORCID,Weninger Clemens³^ORCID,Fuller Franklin⁴^ORCID,Gee Leland⁴^ORCID,Sato Takahiro⁴^ORCID,Seaberg Matthew⁴^ORCID,Alonso-Mori Roberto⁴^ORCID,Bergmann Uwe⁵^ORCID,Yachandra Vittal K.¹^ORCID,Kern Jan¹^ORCID,Yano Junko¹^ORCID

Affiliation:

1. Molecular Biophysics and Integrated Bioimaging Division , Lawrence Berkeley National Laboratory , 1 Cyclotron Road, 94720 , Berkeley , CA , USA

2. Department of Theoretical chemistry and Biology , KTH Royal Institute of Technology , Stockholm , Sweden

3. MAX IV Laboratory , Lund University , Lund , Sweden

4. LCLS, SLAC National Accelerator Laboratory , 94025 , Menlo Park , CA , USA

5. Department of Physics , University of Wisconsin-Madison , 53706 , Madison , WI , USA

Abstract

Abstract X-ray crystallography and X-ray spectroscopy using X-ray free electron lasers plays an important role in understanding the interplay of structural changes in the protein and the chemical changes at the metal active site of metalloenzymes through their catalytic cycles. As a part of such an effort, we report here our recent development of methods for X-ray absorption spectroscopy (XAS) at XFELs to study dilute biological samples, available in limited volumes. Our prime target is Photosystem II (PS II), a multi subunit membrane protein complex, that catalyzes the light-driven water oxidation reaction at the Mn4CaO5 cluster. This is an ideal system to investigate how to control multi-electron/proton chemistry, using the flexibility of metal redox states, in coordination with the protein and the water network. We describe the method that we have developed to collect XAS data using PS II samples with a Mn concentration of <1 mM, using a drop-on-demand sample delivery method.

Funder

National Institutes of Health

U.S. Department of Energy

Publisher

Walter de Gruyter GmbH

Subject

General Chemical Engineering,General Chemistry

Link

https://www.degruyter.com/document/doi/10.1515/pac-2023-0213/pdf

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