Direct-imaging DOEs for high-NA multi-spot confocal microscopy

Author:

Li Zheng12,Taphanel Miro12,Längle Thomas2,Beyerer Jürgen12

Affiliation:

1. Lehrstuhl für Interaktive Echtzeitsysteme (IES), Karlsruher Institut für Technologie (KIT) , Karlsruhe , Germany

2. Fraunhofer-Institut für Optronik, Systemtechnik und Bildauswertung IOSB , Karlsruhe , Germany

Abstract

Abstract Diffractive lens arrays with overlapping apertures can produce spots with high numerical apertures (NAs). Such diffractive optical elements (DOEs) can replace high-NA objectives and measure a large area with high resolution in transmission microscopes. However, in reflection microscopes for surface measurements, the axial resolution is still limited by the objectives. Direct-imaging DOEs are proposed to solve the problem. They can perform high-NA multi-spot measurement in reflection configurations in both lateral and axial directions. Experiments demonstrate a lateral non-vanishing contrast up to 1448 lp/mm and an axial response on a plane mirror with a full width at half maximum (FWHM) of 2.24 μm.

Publisher

Walter de Gruyter GmbH

Subject

Electrical and Electronic Engineering,Instrumentation

Reference9 articles.

1. E. Dai, C. Zhou, P. Xi und L. Liu. Multifunctional doublelayered diffractive optical element. Optics letters, 28(17): 1513–1515, 2003.

2. B. Hulsken, D. Vossen und S. Stallinga. High NA diffractive array illuminators and application in a multi-spot scanning microscope. Journal of the European Optical Society-Rapid publications, 7, 2012.

3. H. Kirshner, D. Sage und M. Unser. 3D PSF models for fluorescence microscopy in ImageJ. In Proceedings of the Twelfth International Conference on Methods and Applications of Fluorescence Spectroscopy, Imaging and Probes (MAF’11), S. 154, Strasbourg, French Republic, September 11-14, 2011.

4. Z. Li. Application of diffractive optical elements in confocal microscopy. In M. Taphanel und J. Beyerer, editors, Proceedings of the 2018 Joint Workshop of Fraunhofer IOSB and Institute for Anthropomatics, Vision and Fusion Laboratory. KIT Scientific Publishing, Karlsruhe, 2019.

5. Z. Li, M. Taphanel, T. Längle und J. Beyerer. Application of DOE in confocal microscopy for surface measurement. In M. Rosenberger, P.-G. Dittrich und B. Zagar, editors, IMEKO Joint TC1 - TC2 International Symposium on Photonics and Education in Measurement Science, Band 11144, S. 254 – 261. International Society for Optics and Photonics, SPIE, 2019.

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