Affiliation:
1. Institute of Resource Biology & Biotechnology, Department of Biotechnology, College of Life Science & Technology, Huazhong University of Science & Technology, Wuhan 430074, P. R. China. Fax: +86-27-87 79 22 65
Abstract
A new full-length β-carbonic anhydrase cDNA was obtained from Brassica napus by homologous cloning. The cDNA has an open-reading frame of 996 nucleotides, encoding 331 amino acids with a calculated molecular weight of 35,692 Da and an estimated pI value of 5.459. The deduced amino acid sequence of β-carbonic anhydrase from Brassica napus shared significant identity with β-carbonic anhydrases from Brassica carinata, Arabidopsis thaliana, and Thlaspi caerulescens (97.9%, 94%, and 93.5% identity, respectively). This cDNA was expressed in Escherichia coli BL21 (DE3) using the expression vector pET-32a(+). The expression band corresponded to the calculated mass plus the N-terminal fusion protein derived from the vector
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
2 articles.
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