Numerical and Experimental Study on a Microfluidic Concentration Gradient Generator for Arbitrary Approximate Linear and Quadratic Concentration Curve Output-Reference-Cited by-同舟云学术

Numerical and Experimental Study on a Microfluidic Concentration Gradient Generator for Arbitrary Approximate Linear and Quadratic Concentration Curve Output

Published:2017-05-11 Issue:1 Volume:16 Page:
ISSN:1542-6580
Container-title:International Journal of Chemical Reactor Engineering
language:en
Short-container-title:

Author:

Chen Xueye¹,Hu Zengliang¹,Zhang Lei¹,Yao Zhen¹,Chen Xiaodong¹,Zheng Yue¹,Liu Yanlin¹,Wang Qing¹,Liu Yang¹,Cui Xuemiao¹,Song Hongxu¹

Affiliation:

1. Faulty of Mechanical Engineer and Automation , Liaoning University of Technology , Jinzhou 121001 , China

Abstract

Abstract This work introduces a simple and versatile method for researching the concentration gradient generator (CGG) which can present the arbitrary approximate linear and quadratic concentration gradient curves output. The concentration gradients of arbitrary approximate linear curves with two inlets and arbitrary quadratic curves in the CGG with three inlets are obtained with the corresponding flow velocities. The CGG was simulated basing on the finite element method (FEM). The fluid-dynamic and mass-transport about the CGG was studied. Moreover, the feasibility of simulation was clearly verified by an experiment which two microfluidic chips of CGG on the PMMA substrate were processed using CNC engraving and milling machine. The paper successfully demonstrates the controllability of concentration gradient profiles in CGG with two inlets and three inlets. The study on the CGG can help the trends study of cell and molecule in different samples in the biochemical engineering.

Publisher

Walter de Gruyter GmbH

Subject

General Chemical Engineering

Link

https://www.degruyter.com/document/doi/10.1515/ijcre-2016-0204/pdf

Reference25 articles.

1. Bombaugh, K.J. 1975. “A Novel Method of Generating Multislope Gradients for High-Pressure Liquid Chromatography.” Journal of Chromatography A 107 (1): 201–206.

2. Cappiello, A., G. Famiglini, C. Fiorucci, F. Mangani, P. Palma, and A. Siviero. 2003. “Variable-Gradient Generator for Micro-And Nano-Hplc.” Analytical Chemistry 75 (5): 1173–1179.

3. Chen, A., J.J. Lu, C. Gu, M. Zhang, and K. B. Lynch. 2015. “Combining Selection Valve and Mixing Chamber for Nanoflow Gradient Generation: Toward Developing a Liquid Chromatography Cartridge Coupled with Mass Spectrometer for Protein and Peptide Analysis.” Analytica Chimica Acta 887: 230–236.

4. Chen, A., K.B. Lynch, X. Wang, et al. 2014. “Incorporating High-Pressure Electroosmotic Pump and a Nano-Flow Gradient Generator into a Miniaturized Liquid Chromatographic System for Peptide Analysis.” Analytica Chimica Acta 844: 90–98.

5. Chen, X., and T. Li. 2016. “A Novel Design for Passive Misscromixers Based on Topology Optimization Method.” Biomedical Microdevices 18 (4): 1–15.

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