A Green Process for Synthesis of Geraniol Esters by Immobilized Lipase from Candida Antarctica B Fraction in Non-Aqueous Reaction Media: Optimization and Kinetic Modeling

Abstract

Abstract Enzymatic synthesis of molecules such as flavors, perfumes and fragrances has a great commercial advantage of being marketed as “natural” and also it offers exquisite selectivity of enzymes that can be superior over chemical catalysis. The current work focuses on the enzymatic synthesis of geranyl acetate as model compound, including optimization of reaction conditions such as nature of catalyst, reaction media, speed of agitation, mole ratio and temperature. A variety of esters were also synthesized. Geraniol was esterified with various acids, aromatic esters and vinyl esters in 1:4 molar ratio. Among all vinyl ester was the best giving in good yield (77–100 %) as compared to aromatic esters (5–82 %) and acids (7–31 %). Novozym 435 was found to be most active catalyst with ~96 % conversion and 100 % selectivity in 60 min at 55 °C in n-heptane as solvent for geranyl acetate. The maximum reaction rate was estimated (Vmax = 0.2712 mol L−1 min-1) by using the double reciprocal plot. It is a ternary complex (ordered bi-bi) mechanism with inhibition by geraniol.