An Isocratic High-Pressure Liquid Chromatographie Purification Method for Radioactively Labeled Deoxyribonucleoside Triphosphates

Abstract

Abstract A method is described for the rapid purification of radioactively labeled deoxyribonucleoside tri­phosphates from their spontaneously emerging hydrolysis products deoxyribonucleoside diphosphate, deoxyribonucleoside monophosphate, and deoxyribonucleoside. The separations which are finished within 3 min or less are carried out on a 0.1X5 cm column filled with LiChrosorb-NH2 , using isocratic elution with 0.025 м potassium phosphate, pH 6 .8 , in a high-pressure liquid chromatograph at room temperature and a flow rate of 30 ml · h-1(flow velocity 63.7 cm·min-1).