Kontakt von Agrobacterium mit Wundrandzellen als Vorbedingung für eine Tumorinduktion / Contact of Agrobacterium with Wound Cells as a Prerequisite of Tumor Induction

Abstract

Tumor induction by Agrobacterium tumefaciens on Kalanchoe leaves is suppressed by charcoal mixed with the inoculum. The inhibition works best with a certain mass relation between bacteria and charcoal. The bacteria become adsorbed to the charcoal particles and in this state they are unable to induce tumors. If charcoal is added to bacteria-infected wounds only 15 min after infection it is not interfering with tumor formation. These results are supporting the hypothesis of sites in the wound to which Agrobacterium has to be attached to incite tumors.