Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals. II. Vacuolar Localization of Some Hydrolases-Reference-Cited by-同舟云学术

Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals. II. Vacuolar Localization of Some Hydrolases

Published:1985-04-01 Issue:3-4 Volume:40 Page:196-200
ISSN:1865-7125
Container-title:Zeitschrift für Naturforschung C
language:en
Short-container-title:

Author:

Aerts J. M. F. G.¹,Schram A. W.¹

Affiliation:

1. Departments of Genetics and Plant Physiology, Genetisch Instituut, Kruislaan 318, 1098 SM Amsterdam /The Netherlands

Abstract

In vacuoles isolated from petal upper epidermis of Petunia hybrida through polybase-induced lysis of protoplasts the vacuolar localization of α-mannosidase and α-galactosidase was demonstrated. No association was observed between the tonoplast and these hydrolases. Protoplasts from different mutant lines blocked in anthocyanin biosynthesis were treated with DEAE-dextran to release vacuoles. The differences in quality of the obtained vacuolar preparations indicated the relation between mutant line used and successful application of the polybase-procedure.

Publisher

Walter de Gruyter GmbH

Subject

General Biochemistry, Genetics and Molecular Biology

Link

https://www.degruyter.com/document/doi/10.1515/znc-1985-3-410/pdf

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