Embryonale Hämoglobine der Säuger: Die Sequenzen der ζ-, ε-und ϑ-Ketten vom Hausschwein (Sus scrofa domestica) Embryonic Hemoglobins in Mammals: The Primary Structures of e-and 5-Chains of the Pig (Sus scrofa dom estica) / Embryonic Hemoglobins in Mammals: The Primary Structures of ζ-, ε-and ϑ-Chains of the Pig (Sms scrofa domesrica)

Abstract

The amino-acid sequences of all expressed hemoglobins of the pig embryo are given: Hemo­globin Gower I (ζ2/ε2), Hemoblobin Gower II (α2/ε2), Hemoglobin Heide I (ζ2/ϑ2) and Hemo­globin Heide II (α0/ϑ2). The ζ-, ε- and ϑ-chains were obtained with chromatography on CM-cellulose from isolated hemoglobin components. The primary structure was established by sequencing the tryptic peptides in the sequenator: they where isolated using HPLC. The ζ-chains from pig and human differ in 23, the ε-chains in 20 positions. The embryonic globin-gene which express the ϑ-chains, is a new one in mammals, of ε-type and up to now it could only be found in pigs: the amino-acid sequence differ in only 4 positions from the ε-chains. Because no γ-chains (fetal Hb) are expressed the sequences of all hemoglobins (5 hemoglobin chains forming 5 different hemo­globins) of ontogeny in pig are now described.