Synthesis of 197m/gHg labelled gold nanoparticles for targeted radionuclide therapy
Author:
Droop Philipp12, Chen Shaohuang13, Radford Melissa J.3, Paulssen Elisabeth24, Gates Byron D.3, Reilly Raymond M.5, Radchenko Valery16, Hoehr Cornelia1
Affiliation:
1. Life Sciences Division , TRIUMF , Vancouver , BC V6T 2A3, 4004 Wesbrook Mall , Canada 2. Faculty of Chemistry and Biotechnology , FH Aachen , 52428 Jülich , Heinrich-Mußmann-Straße 1 , Germany 3. Department of Chemistry , Simon Fraser University , Burnaby , BC V5A 1S6, 8888 University Dr , Canada 4. Department of Radiation Science and Technology , TU Delft , 2698 JB Delft, Mekelweg 15 , Delft , The Netherlands 5. Leslie Dan Faculty of Pharmacy , University of Toronto , Toronto , ON M5S 1A1, 144 College St , Canada 6. Department of Chemistry , University of British Columbia , Vancouver , V6T 1Z4 , Canada
Abstract
Abstract
Meitner-Auger-electron emitters have a promising potential for targeted radionuclide therapy of cancer because of their short range and the high linear energy transfer of Meitner-Auger-electrons (MAE). One promising MAE candidate is 197m/gHg with its half-life of 23.8 h and 64.1 h, respectively, and high MAE yield. Gold nanoparticles (AuNPs) that are labelled with 197m/gHg could be a helpful tool for radiation treatment of glioblastoma multiforme when infused into the surgical cavity after resection to prevent recurrence. To produce such AuNPs, 197m/gHg was embedded into pristine AuNPs. Two different syntheses were tested starting from irradiated gold containing trace amounts of 197m/gHg. When sodium citrate was used as reducing agent, no 197m/gHg labelled AuNPs were formed, but with tannic acid, 197m/gHg labeled AuNPs were produced. The method was optimized by neutralizing the pH (pH = 7) of the Au/197m/gHg solution, which led to labelled AuNPs with a size of 12.3 ± 2.0 nm as measured by transmission electron microscopy. The labelled AuNPs had a concentration of 50 μg (gold)/mL with an activity of 151 ± 93 kBq/mL (197gHg, time corrected to the end of bombardment).
Funder
NRC NSERCDiscovery Program Canadian Cancer SocietyInnovation Grant
Publisher
Walter de Gruyter GmbH
Subject
Physical and Theoretical Chemistry
Reference18 articles.
1. Filosofov, D., Kurakina, E., Radchenko, V. Potent candidates for targeted auger therapy: production and radiochemical considerations. Nucl. Med. Biol. 2021, 94, 1; https://doi.org/10.1016/j.nucmedbio.2020.12.001. 2. Koning, A. J., Rochman, D., Sublet, J. C., Dzysiuk, N., Fleming, M., van der Marck, S. TENDL: complete nuclear data library for innovative nuclear science and technology. Nucl. Data Sheets 2019, 155, 1; https://doi.org/10.1016/j.nds.2019.01.002. 3. Huang, X., Zhou, C. Nuclear data sheets for A = 197. Nucl. Data Sheets 2005, 104, 283; https://doi.org/10.1016/j.nds.2005.01.001. 4. Ku, A., Facca, V. J., Cai, Z., Reilly, R. Auger electrons for cancer therapy–a review. EJNMMI Radiopharm. Chem. 2019, 4, 1; https://doi.org/10.1186/s41181-019-0075-2. 5. Parsons, D. W., Jones, S., Zhang, X., Lin, J. C. H., Leary, R. J., Angenendt, P., Mankoo, P., Carter, H., Siu, I. M., Gallia, G. L., Olivi, A., McLendon, R., Rasheed, B. A., Keir, S., Nikolskaya, T., Nikolsky, Y., Busam, D. A., Tekleab, H., Diaz, L. A., Hartigan, J., Smith, D. R., Strausberg, R. L., Marie, S. K. N., Shinjo, S. M. O., Yan, H., Riggins, G. J., Bigner, D. D., Karchin, R., Papadopoulos, N., Parmigiani, G., Vogelstein, B., Velculescu, V. E., Kinzler, K. W. An integrated genomic analysis of human glioblastoma multiforme. Science 2008, 321, 1807; https://doi.org/10.1126/science.1164382.
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