Impact of gamma irradiation on phytochemical composition, and biological activities of Lepidium sativum seeds extract
Author:
Hadj Rabia Samia1, Debib Aicha2, Eddaikra Atika3, Aberkane-Mchebbek Lila4, Nouri Ramy1, Benmoussa Fatima1, Mokhtari Amine1, Medjber Sarah1, Mansouri Belkacem5, Messaoudi Mohammed6ORCID
Affiliation:
1. Department of Nuclear Applications , Nuclear Research Center of Draria , B.P.43 Sebala-Draria , Algeria 2. Laboratory of Management and Valorization of Agricultural and Aquatic Ecosystems (LMVAAE) , University Center of Tipaza Morsli Abdallah , Oued Merzoug , Tipaza 42022 , Algeria 3. Bioinformatics , Molecular Modeling and Drug Design M’Hamed Bougara University , Boumerdes , Algeria 4. Department of Radiobiology and Life Sciences , Nuclear Research Center of Algiers , BP-399 Frantz Fanon , Algeria 5. Department of Operational Exploitation and Maintenance of the Experimental Platform , Nuclear Research Center of Algiers , BP-399 Frantz Fanon , Algeria 6. Nuclear Research Centre of Birine , P.O. Box 180, Ain Oussera , Djelfa 17200 , Algeria
Abstract
Abstract
The main objective of this research is to assess how gamma radiation influences the chemical composition and activities such as antioxidant, antimicrobial and anticancer activities of Lepidium sativum (L. sativum) seeds. Methanolic extracts were obtained through a 24 h maceration process from L. sativum seeds exposed to various doses of gamma irradiation. Phytochemical screening involved precipitation and staining reactions, with the antioxidant potential assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Antimicrobial activity was evaluated through a diffusion test on agar medium, while the cytotoxic potential on the lung cancer line “A-549 cells” was examined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Qualitative analysis of the extracts identified preserved bioactive constituents after irradiation. Additionally, quantitative analysis indicated a significant rise in the total content of polyphenols and flavonoids under radiation, accompanied by an enhanced reducing power compared to the control. The antimicrobial and cytotoxic capabilities also improved, evident in increased growth inhibition zones for certain microbial strains and reduced viability rates of A549 cells. These results show promise and warrant further investigation into the potential of gamma radiation to enhance additional biological effects of plants.
Publisher
Walter de Gruyter GmbH
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