Complexation of Cm(III) with blood serum proteins: recombinant human serum albumin (rHSA)
Author:
Adam Nicole1, Reitz Cédric Y.1, Ditter Anna-Lena2, Panak Petra J.21
Affiliation:
1. Institute for Nuclear Waste Disposal (INE) , Karlsruhe Institute of Technology (KIT) , P.O. Box 3640 , 76021 Karlsruhe , Germany 2. Institute of Physical Chemistry, University of Heidelberg , Im Neuenheimer Feld 253 , 69120 Heidelberg , Germany
Abstract
Abstract
The complexation of Cm(III) with the recombinant human serum albumin (rHSA) (characterized by single deletion of residue Asp-1), is studied in dependence of pH and rHSA concentration using time-resolved laser fluorescence spectroscopy (TRLFS). A Cm(III) rHSA species is formed between pH 6.4 and 10.0 with the conditional stability constant being logK = 6.47 at pH = 7.4. Competition titration experiments with Cu(II) and Zn(II) confirm complexation at the N-terminal binding site (NTS) of rHSA and exclude the involvement of the Multi-Metal Binding Site (MBS). Comparison with a previous study on Cm(III) interaction with native albumin, HSA, points out, that residue Asp-1 is involved in Cm(III) binding to HSA but is not crucial for Cm(III) complexation at the NTS. The results are of major importance for a better understanding of fundamental actinide-protein interaction mechanisms which are highly required for the identification and characterization of relevant distribution pathways of incorporated radionuclides.
Publisher
Walter de Gruyter GmbH
Subject
Physical and Theoretical Chemistry
Reference29 articles.
1. Ansoborlo, E., Bion, L., Doizi, D., Moulin, C., Lourenco, V., Madic, C., Cote, G., Van der Lee, J., Moulin, V. Current and future radionuclide speciation studies in biological media. Radiat. Protect. Dosim. 2007, 127, 97; https://doi.org/10.1093/rpd/ncm258. 2. Gorden, A. E. V., Xu, J. D., Raymond, K. N., Durbin, P. Rational design of sequestering agents for plutonium and other actinides. Chem. Rev. 2003, 103, 4207; https://doi.org/10.1021/cr990114x. 3. Sugio, S., Kashima, A., Mochizuki, S., Noda, M., Kobayashi, K. Crystal structure of human serum albumin at 2.5 angstrom resolution. Protein Eng. 1999, 12, 439; https://doi.org/10.1093/protein/12.6.439. 4. He, X. M., Carter, D. C. Atomic-structure and chemistry of human serum-albumin. Nature 1992, 358, 209; https://doi.org/10.1038/358209a0. 5. Bal, W., Sokolowska, M., Kurowska, E., Faller, P. Binding of transition metal ions to albumin: sites, affinities and rates. Biochim. Biophys. Acta Gen. Subj. 2013, 1830, 5444.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|