Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization
Author:
Erol Kadir1ORCID, Tatar Demet2, Veyisoğlu Aysel3, Tokatlı Ali4
Affiliation:
1. Department of Medical Services and Techniques , Vocational School of Health Services, Hitit University , Çorum , Turkey 2. Department of Medical Services and Techniques , Osmancık Ömer Derindere Vocational School, Hitit University , Çorum , Turkey 3. Department of Medical Services and Techniques , Vocational School of Health Services, Sinop University , Sinop , Turkey 4. Department of Biology , Faculty of Art and Science, Ondokuz Mayıs University , Samsun , Turkey
Abstract
Abstract
Micron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 μg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.
Publisher
Walter de Gruyter GmbH
Subject
Materials Chemistry,Polymers and Plastics,General Chemical Engineering
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