Quantitative assessment of antioxidant potential of selected homeopathic preparations in clinical practice
Author:
Kumar Arun12ORCID, Prajapati Suneel1, Sharma Mahima1, Singh Tejveer3, Choudhary Neha4, Bharti Alok C.5, Sharma Rohit6ORCID, Gupta Pankaj1
Affiliation:
1. Molecular Biology Laboratory, Drug Standardization Unit , Dr. DP Rastogi Central Research Institute of Homeopathy, Ministry of AYUSH, Government of India , Noida 201301 , Uttar Pradesh , India 2. Department of Zoology , Kirori Mal College , University of Delhi , Delhi - , India 3. Department of Zoology , Hansraj College , University of Delhi , Delhi- , India 4. Centre for Computational Biology and Bioinformatics , Central University of Himachal Pradesh , Dharamshala , Himachal Pradesh , India 5. Department of Zoology, Molecular Oncology Laboratory , University of Delhi , Delhi 110007 , India 6. Department of Rasa Shastra and Bhaishajya Kalpana, Faculty of Ayurveda, Institute of Medical Sciences , Banaras Hindu University , Varanasi , Uttar Pradesh 221005 , India
Abstract
Abstract
Objectives
Antioxidant property like radical scavenging is a primary target to elucidate the efficacy mechanism of a drug against diseases linked to oxidative stress such as cancer, metabolic disorders, rheumatoid arthritis, etc. In alternative therapies, homeopathy is one of the preferred choices by patients and clinicians due to its potential to cure chronic and complex illnesses. However, the efficacy of homeopathic preparations at high diluted potencies attracts rational criticism due to insufficient scientific knowledge supporting the mechanism of action. Therefore, an attempt was made to estimate the total phenolic content (TPC) and radical scavenging activity of clinically prescribed homeopathic drugs.
Methods
With gallic acid as a reference control, mother tinctures (MTs) and different potencies of Eucalyptus globulus (EG), Syzygium jambolanum (SJ), Ruta graveolens (RG), and Thuja occidentalis (TO) were used to perform Folin–Ciocalteu test, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.
Results
The results showed TPC of MTs equivalent to µg/mL of gallic acid viz; EG (4,872.5 ± 133.2), SJ (8,840.5 ± 14.8), RG (985.6 ± 39.1), and TO (341.5 ± 19.5) with significant ABTS and DPPH radical scavenging potential. Whereas 30C and 200C potencies of each homeopathic drug showed undetectable phenolic content and insignificant radical scavenging potential compared to vehicle control, i.e., alcohol 90% (2.0 ± 1.5).
Conclusions
The reported efficacy of 30C and 200C potencies of homeopathic medicines against oxidative stress-related illnesses might be due to mechanisms other than radical scavenging. Furthermore, the assays studied can be helpful in drug standardization and quality control of MTs that are used as starting material in homeopathic preparations.
Funder
Central Council for Research in Homoeopathy (CCRH) Ministry of AYUSH, Govt. of India
Publisher
Walter de Gruyter GmbH
Subject
Pharmacology (medical),General Pharmacology, Toxicology and Pharmaceutics
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