A guide to designing photocontrol in proteins: methods, strategies and applications
Author:
Kneuttinger Andrea C.1ORCID
Affiliation:
1. Institute of Biophysics and Physical Biochemistry and Regensburg Center for Biochemistry, University of Regensburg , D-93040 Regensburg , Germany
Abstract
Abstract
Light is essential for various biochemical processes in all domains of life. In its presence certain proteins inside a cell are excited, which either stimulates or inhibits subsequent cellular processes. The artificial photocontrol of specifically proteins is of growing interest for the investigation of scientific questions on the organismal, cellular and molecular level as well as for the development of medicinal drugs or biocatalytic tools. For the targeted design of photocontrol in proteins, three major methods have been developed over the last decades, which employ either chemical engineering of small-molecule photosensitive effectors (photopharmacology), incorporation of photoactive non-canonical amino acids by genetic code expansion (photoxenoprotein engineering), or fusion with photoreactive biological modules (hybrid protein optogenetics). This review compares the different methods as well as their strategies and current applications for the light-regulation of proteins and provides background information useful for the implementation of each technique.
Publisher
Walter de Gruyter GmbH
Subject
Clinical Biochemistry,Molecular Biology,Biochemistry
Reference325 articles.
1. Acosta-Ruiz, A., Gutzeit, V.A., Skelly, M.J., Meadows, S., Lee, J., Parekh, P., Orr, A.G., Liston, C., Pleil, K.E., Broichhagen, J., et al.. (2020). Branched photoswitchable tethered ligands enable ultra-efficient optical control and detection of G protein-coupled receptors in vivo. Neuron 105: 446–463, https://doi.org/10.1016/j.neuron.2019.10.036. 2. Agarwal, P.K., Schultz, C., Kalivretenos, A., Ghosh, B., and Broedel, S.E. (2012). Engineering a hyper-catalytic enzyme by photoactivated conformation modulation. J. Phys. Chem. Lett. 3: 1142–1146, https://doi.org/10.1021/jz201675m. 3. Agostini, F., Völler, J.-S., Koksch, B., Acevedo-Rocha, C.G., Kubyshkin, V., and Budisa, N. (2017). Biocatalysis with unnatural amino acids. Enzymology meets xenobiology. Angew. Chem. Int. Ed. 56: 9680–9703, https://doi.org/10.1002/anie.201610129. 4. Agostinis, P., Berg, K., Cengel, K.A., Foster, T.H., Girotti, A.W., Gollnick, S.O., Hahn, S.M., Hamblin, M.R., Juzeniene, A., Kessel, D., et al.. (2011). Photodynamic therapy of cancer. An update. CA-Cancer J. Clin. 61: 250–281, https://doi.org/10.3322/caac.20114. 5. Algorri, J.F., Ochoa, M., Roldán-Varona, P., Rodríguez-Cobo, L., and López-Higuera, J.M. (2021). Photodynamic therapy: a compendium of latest reviews. Cancers 13: 4447, doi:https://doi.org/10.3390/cancers13174447.
Cited by
12 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|