GC–MS analysis of <i>Lactobacillus plantarum</i> YW11 metabolites and its computational analysis on familial pulmonary fibrosis hub genes-Reference-Cited by-同舟云学术

GC–MS analysis of Lactobacillus plantarum YW11 metabolites and its computational analysis on familial pulmonary fibrosis hub genes

Published:2024-01-01 Issue:1 Volume:22 Page:
ISSN:2391-5420
Container-title:Open Chemistry
language:en
Short-container-title:

Author:

Naveed Muhammad¹,Jamil Hamza¹,Aziz Tariq²³,Makhdoom Syeda Izma¹,Sarwar Abid²,Nasbeeb Jasra²,Zhennai Yang²,Alharbi Metab⁴

Affiliation:

1. Department of Biotechnology, Faculty of Science and Technology, University of Central Punjab , Lahore , 54590 , Pakistan

2. Key Laboratory of Geriatric Nutrition and Health of Ministry of Education, Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University , No. 11 Fucheng Road, Hai-Dian District , Beijing , 100048 , China

3. Laboratory of Animal Health, Food Hygiene and Quality, Department of Agriculture, University of Ioannina , 47132 , Arta , Greece

4. Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University , P.O. Box 2455 , Riyadh , 11451 , Saudi Arabia

Abstract

Abstract The purpose of this research was to examine the interaction between metabolites of Lactobacillus plantarum YW11, characterized through GC–mass spectra (MS) analysis, and the FN1 protein in cases of familial pulmonary fibrosis, found from hub genes analysis. GC–MS analysis was performed to identify metabolites in L. plantarum. Then, gene expression analysis and functional annotations were conducted to investigate the hub genes. A network of hub genes and transcription factors (TFs) was constructed, highlighting the significance of FN1 in the disease’s etiology. Molecular docking was employed to explore the interaction between the characterized metabolites and the FN1 protein. Toxicity analysis was also carried out. Thirty-two active compounds of L. plantarum YW11 were characterized by GC–MS. The gene expression analysis identified 295 differentially expressed genes, including 10 hub genes and 6 TFs, providing further support for the involvement of FN1 protein in the disease. The results of the molecular docking studies suggest the therapeutic potential of targeting FN1, with the best docking result observed for the interaction between FN1 and the 2,4-di-tert-butylphenol metabolite (energy of −6.9 kcal/mol). The toxicity analysis and molecular dynamic simulations support the suitability of 2,4-di-tert-butylphenol as a candidate for targeting FN1.

Publisher

Walter de Gruyter GmbH

Link

https://www.degruyter.com/document/doi/10.1515/chem-2024-0019/pdf

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