Involvement of the Superoxide Free Radical Ion in Photosynthetic Oxygen Reduction

Abstract

Abstract Photosynthetic oxygen reduction by isolated chloroplast lamellar systems has been studied with the aid of superoxide dismutase. Two mechanisms of oxygen reduction by illuminated chloroplast lamellar systems can be differentiated: 1. In the presence of low potential electron acceptors like AQ or MV the superoxide free radical ion is the product of autooxidation of the reduced acceptor. Addition of superoxide dismutase has no influence on the initial rates of oxygen reduction. 2. Stimulation of photosynthetic oxygen reduction by o-diphenols is only observed in the absence of superoxide dismutase and ascorbate; apparently the superoxide free radical ions is involved in both initiation and propagation of a chain reaction. If the ferredoxin-stimulated photosynthetic oxygen reduction is measured, both ascorbate and superoxide dismutase are active as inhibitors. By heating suspensions of chloroplast lamellar systems, a substance is released into the supernatant which exhibits the activity of an oxygen reducing factor (ORF) with properties similar to o-diphenols: The stimulation of photosynthetic oxygen reduction is reversed by addition of either SDM or ascorbate. A reaction sequence for photosynthetic oxygen reduction in the presence of ferredoxin is considered, which is initiated by the superoxide free radical ion produced by autooxidation of reduced ferredoxin; the superoxide free radical ion "activates" an endogenous oxygen reducing factor, which in this "active" state can reduce oxygen to O2·-. The presence of either superoxide dismutase or ascorbate yields in chain termination by scavenging the superoxide free radical ion