Der Einfluß von 4-Hydroxy-2.3-pentenal (HPE) auf die Vermehrung von Vaccinia-Virus in Hühnerfibroblastenkulturen / Influence of 4-Hydroxy-2,3-pentenale (HPE) on the Multiplication of Vaccinia-virus in Chick-embryo Fibroblast Cultures

Abstract

Abstract The influence of 4-hydroxy-2,3-/rans-pentenale (HPE) on multiplication of Vaccinia virus in chick fibroblast cultures has been investigated. The cytotoxicity of HPE has been estimated by in ­ corporation of [3H] thymidine, [3H] uridine, [3H] leucine and by morphological tests. The greatest influence of H PE was seen after incorporation of [3H] thymidine. Chick-embryo cultures infected with Vaccinia virus showed a marked increase of DNA synthesis in contrast to the controls. This increase averaged 180% after 16 to 14 hours. HPE 0.05-10_ 3 M caused a reduction of 72% in [3H] thymidine incorporation in virus infected cultures. At the same time virus concentration decreased by 76%. HPE 0.1-10~3 M, a concentration which inhibits the normal cell DNA synthesis by 50%, caused about 90% inhibition in infected cultures; virus m ulti­ plication was 99% inhibited. These findings have been attributed to a selective inhibition of virus DNA synthesis by HPE. and possible mechanisms are discussed.