Imaging of native early embryogenic tissue of Scots pine (Pinus sylvestris L.) by ESEM

Author:

Hřib Jiří1,Vooková Božena2,Neděla Vilém1

Affiliation:

1. 1Institute of Scientific Instruments, Academy of Sciences of the Czech Republic, CZ-612 64 Brno, Czech Republic

2. 2Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, SK-95007 Nitra, Slovak Republic

Abstract

AbstractEnvironmental scanning electron microscopy enables the investigation of uncoated pine early embryogenic tissue samples in situ. The samples were examined under low vacuum conditions (air pressure 550 Pa) at a temperature of around -18°C by the AQUASEM II noncommercial environmental scanning electron microscope. The native extracellular matrix surface network was imaged by the environmental scanning electron microscope and in dark field mode of the optical microscope too. The backscattered electron detector disclosed brightness loci in the cells of early embryogenic culture. This work shows images of native pine embryogenic tissues. The continuity of extracellular matrix with structural integrity of plant organism is discussed.

Publisher

Walter de Gruyter GmbH

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Reference17 articles.

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5. von Effect of parent genotype on somatic embryogenesis of Scots pine ( Pinus sylvestris );Niskanen;Tree Physiol,2004

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