A new combination of substrates: biogas production and diversity of the methanogenic microorganisms

Author:

Kushkevych Ivan1,Vítězová Monika2,Vítěz Tomáš3,Kováč Jozef2,Kaucká Petra4,Jesionek Wojciech5,Bartoš Milan2,Barton Larry6

Affiliation:

1. Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 753/5, 62500Brno, Czech Republic

2. Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic

3. Department of Agricultural, Food and Environmental Engineering Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic

4. Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic

5. Institute of Biophysics of the CAS, Department of Plant Developmental Genetics, Brno, Czech Republic

6. Department of Biology, Laboratory of Microbial Chemistry, University of New Mexico, New Mexico, USA

Abstract

AbstractAgriculture, food industry, and manufacturing are just some of the areas where anaerobic technology can be used. Currently, anaerobic technologies are mainly used for wastewater treatment, solid waste treatment, or for the production of electrical and thermal energy from energy crops processing. However, a clear trend is towards more intensive use of this technology in biomass and biodegradable waste processing and hydrogen or biomethane production. An enormous number of anaerobic digesters are operating worldwide but there is very little information about the effect of different substrate combinations on the methanogens community. This is due to the fact that each of the anaerobic digesters has its own unique microbial community. For the most effective management of anaerobic processes it would be important to know the composition of a consortium of anaerobic microorganisms present in anaerobic digesters processing different input combinations of raw material. This paper characterizes the effect of the input raw materials on the diversity of the methanogen community. Two predominant microorganisms in anaerobic digesters were found to be 99% identity by the sequences of the 16S rRNA gene to theMethanoculleusandThermogymnomonasgenera deposited in GenBank.

Publisher

Walter de Gruyter GmbH

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

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