Action of fungal β-glucosidase on the mono-O-methylated p-nitrophenyl β-d-glucopyranoside 2nd ICC 2007, Tokyo, Japan, October 25–29, 2007

Abstract

AbstractThe action of β-glucosidase on the methyl derivatives ofp-nitrophenyl β-d-glucopyranoside (pNP-β-Glc), which were regio-specifically substituted atO-2,O-3,O-4, andO-6positions, was studied. Specifically, several β-glucosidases isolated from brown-rot, white-rot, soft-rot fungi, and almond were investigated. These β-glucosidases did not act on the 2, 3, and 4-O-methyl derivatives, while the 6-O-methyl one was hydrolyzed by all the enzymes to some extent. The results indicate that the methyl group atO-2,O-3, andO-4of the glucopyranoside strongly inhibits the recognition by the β-glucopyranoside, while the enzymes do not discriminate the structure difference betweenpNP-β-Glc and its methyl derivative atO-6.