Vergleichende Untersuchungen über die Protein- und Nucleinsäuresynthese in Tumorzellen, Embryonen und Retina bei Aerobiose und Anaerobiose

Abstract

The rate of incorporation of 14CO2 and 4-14C-Aspartic acid in low molecular intermediates, proteins and nucleic acids of ascites tumor cells, Xenopus and chicken embryos and the retina of adult rats was investigated under different conditions. By Ehrlich Ascites tumor cells amino acids are incorporated in the protein fraction aerobically and anaerobically with about the same speed. In embryos the protein synthesis is greatly diminished anaerobically. To compare the efficiency of energy produced by respiration and by glycolysis for incorporation of amino acids in proteins an efficiency coefficient was calculated. For tumor cells this coefficient is anaerobically greater than aerobically, for chicken embryos aerobically greater than anaerobically. The incorporation of 14CO2 in nucleic acids of embryos is more inhibited than the incorporation in the protein fraction. Also the nucleic acid synthesis of tumor cells is inhibited anaerobically. In retina the rate of synthesis of proteins and nucleic acids is much lower compared with embryos and tumor cells aerobically and anaerobically. Glucose being absent only small amounts of 14CO2 were incorporated in the low molecular intermediates, the nucleic acid fraction and protein fraction of tumor cells. Tumor cells respiring only have a smaller efficiency coefficient, than cells respiring and glycolysing.