Analysis of European Honeybee (Apis Mellifera) Wings Using ATR-FTIR and Raman Spectroscopy: A Pilot Study-Reference-Cited by-同舟云学术

Analysis of European Honeybee (Apis Mellifera) Wings Using ATR-FTIR and Raman Spectroscopy: A Pilot Study

Published:2017-03-01 Issue:1 Volume:48 Page:22-29
ISSN:1805-9430
Container-title:Scientia Agriculturae Bohemica
language:en
Short-container-title:

Author:

Machovič V.¹²,Lapčák L.²,Havelcová M.²,Borecká L.²,Novotná Mi.³,Novotná Ma.³,Javůrková I.³,Langrová I.⁴,Hájková Š.⁴,Brožová A.⁴,Titěra Dalibor⁵⁶

Affiliation:

1. University of Chemistry and Technology Prague, Prague, Czech Republic a

2. Institute of Rock Structure and Mechanics of the Czech Academy of Sciences, Prague, a

3. University of Chemistry and Technology Prague, Prague, a

4. Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Prague, a

5. Bee Research Institute, Dol, Czech Republic a

6. Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Zoology and Fisheries, Kamýcká 129, 165 00 Prague-Suchdol, Prague, a

Abstract

Abstract The infrared (ATR-FTIR) and Raman spectroscopy was used for the structural characterization of honeybee wings. Protein, chitin, and lipid characteristic spectral features were detected using both methods. The protein secondary structure was predominantly composed of the β-sheet molecular conformation with β-turns or coil contributions. The vibration modes of the side-chain aromatic amino acid residues (tyrosine, phenylalanine, tryptophan) occurred in the wing spectra. The results of discriminant analysis showed that the infrared spectroscopy of the wing in combination with a multivariate analysis seemed promising for a resolution of the chemical structure of the wings based on lipid, proteins, and chitin content.

Publisher

Walter de Gruyter GmbH

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