Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and <i>in vivo</i> toxicity studies in zebrafish-Reference-Cited by-同舟云学术

Enhanced apoptotic activity of Pluronic F127 polymer-encapsulated chlorogenic acid nanoparticles through the PI3K/Akt/mTOR signaling pathway in liver cancer cells and in vivo toxicity studies in zebrafish

Published:2023-01-01 Issue:1 Volume:23 Page:
ISSN:1618-7229
Container-title:e-Polymers
language:en
Short-container-title:

Author:

Alanazi Fehaid¹,Elderdery Abozer Y.²,Alzahrani Badr²,Alzerwi Nasser A. N.³,Althobiti Maryam Musleh⁴,Rayzah Musaed³,Alanazi Abdulaziz Suailem⁵,Kuriri Fahd A.⁴,Idrees Bandar⁶,Alenazy Fawaz O.²,Alsultan Afnan⁷,Rayzah Fares⁸,Baksh Yaser⁹,Subbiah Suresh Kumar¹⁰,Mok Pooi Ling¹¹

Affiliation:

1. Department of Clinical Laboratory Sciences, College of Applied Medical Sciences-AlQurayyat, Jouf University , Sakaka , Saudi Arabia

2. Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University , Sakaka , Saudi Arabia

3. Department of Surgery, College of Medicine, Majmaah University , P. O. Box 66 , Al-Majmaah 11952 , Riyadh , Kingdom of Saudi Arabia

4. Department of Clinical Laboratory Sciences, College of Applied Medical Sciences , Shaqra University, Shaqra , Riyadh Province 15572 , Saudi Arabia

5. Pathology and Clinical Laboratory Medicine Administration - Flow Cytometry, King Fahad Medical City , Riyadh , Saudi Arabia

6. Department of Surgery, Prince Sultan Military Medical City in Riyadh , Makkah Al Mukarramah Rd , As Sulimaniyah , Saudi Arabia

7. Department of Surgery, King Saud Medical City , Riyadh 12746 , Saudi Arabia

8. Department of Surgery, Aseer Central Hospital , Abha 62523 , Saudi Arabia

9. Department of Surgery, Iman General Hospital , Riyadh 12684 , Saudi Arabia

10. Centre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research , Chennai , India

11. Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia , 43400 UPM Serdang , Selangor , Malaysia

Abstract

Abstract In this study, chlorogenic acid nanoparticles encapsulated in Pluronic F127 polymer were synthesized and characterized to determine if they could treat human liver cancer. The nanoparticles were synthesized using standard procedures and characterized using physical and biological techniques such as X-ray diffraction, Fourier transform infrared spectroscopy, UV-Vis, dynamic light scattering, Photoluminescence, scanning electron microscopy, and transmission electron microscopy. The anticancer effects were assessed using MTT analysis, acridine orange/ethidium bromide, reactive oxygen species (ROS), COMET assay, annexin-V/FITC, cell cycle analysis, and expression of marker genes against HepG2 cell lines. The results showed significant cytotoxicity, apoptosis induction, and increased ROS production in treated cells compared to control cells. The nanoparticles also activated the apoptotic cascade and regulated the PI3K/AKT/mTOR pathways. The nanocomposites exhibited unique characteristics such as anticancer efficacy in vitro. Further research was conducted using zebrafish to model hematological parameters, liver enzymes, and histopathology to study effectiveness. Green-synthesized Pluronic F127–chlorogenic acid nanoparticles can be considered potential cancer therapy agents.

Publisher

Walter de Gruyter GmbH

Subject

Polymers and Plastics,Physical and Theoretical Chemistry,General Chemical Engineering

Link

https://www.degruyter.com/document/doi/10.1515/epoly-2023-0053/pdf

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