Protein and genetic expression of CDKN1C and IGF2 and clinical features related to human umbilical cord length-Reference-Cited by-同舟云学术

Protein and genetic expression of CDKN1C and IGF2 and clinical features related to human umbilical cord length

Published:2020-05-01 Issue:2 Volume:49 Page:229-236
ISSN:1619-3997
Container-title:Journal of Perinatal Medicine
language:en
Short-container-title:

Author:

Olaya-C Mercedes¹^ORCID,Ayala-Ramirez Paola²,Sanchez-Barbero Ana I.³,Guzmán-P Sandra L.⁴,Gil Fabian⁵,Silva Jaime L.⁶,Bernal Jaime E.⁷

Affiliation:

1. Associate Professor, Department of Pathology, The Medical School , Pontificia Universidad Javeriana – Hospital Universitario San Ignacio , Kra 7 40-62 , Bogota , Colombia

2. Institute of Human Genetics, The Medical School , Pontificia Universidad Javeriana , Bogota , Colombia

3. Pontificia Universidad Javeriana – Department of Resident of Human Genetics , Hospital Universitario San Ignacio , Bogota , Colombia

4. Pontificia Universidad Javeriana – Department of Pathology Resident , Hospital Universitario San Ignacio , Bogota , Colombia

5. Universidad Javeriana, Department of Clinical Epidemiology and Biostatistics , Bogota , Colombia

6. Pontificia Universidad Javeriana – San Ignacio University Hospital , Bogota , Colombia

7. Facultad de Salud, Universidad del Sinu , Cartagena , Colombia

Abstract

Abstract Background Umbilical cord (UC) abnormalities are related to neurological outcome and death; specific molecular factors that might be involved are, as yet, unknown; however, protein-coding genes insulin-like growth factor 2 (IGF2) and cyclin-dependent kinase inhibitor 1C (CDKN1C) have been identified as potential candidates. Methods An analytical observational study was carried out. Newborn UCs were collected, along with their clinical and morphological features. Immunohistochemical analysis was made on paraffin-embedded sections and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed in fresh UC tissue for the assessment of gene expression. Results A total of 100 newborns were included. A significant association was found between long UC and prematurity [odds ratio (OR) 9] and long UC and respiratory distress (OR 4.04). Gestational diabetes (OR 8.55) and hypertensive disorders of pregnancy (HDP) (OR 4.71) were found to be related to short UCs. The frequency for abnormal UC length was higher than expected. UC length was positively correlated with maternal, newborn and placental weight. No statistical association was found between IGF2 and CDKN1C (p57) expression and UC length; however, there was a tendency for higher CDKN1C expression in short UCs, while, on the contrary, higher IGF2 expression for long UCs. Conclusion UC length was observed to be associated with maternal and newborn complications. Protein expression, messenger RNA (mRNA) activity and the activity of said genes seem to be related to UC length.

Publisher

Walter de Gruyter GmbH

Subject

Obstetrics and Gynecology,Pediatrics, Perinatology and Child Health

Link

https://www.degruyter.com/document/doi/10.1515/jpm-2019-0442/pdf

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