Membrane-anchored substrate binding proteins are deployed in secondary TAXI transporters-Reference-Cited by-同舟云学术

Membrane-anchored substrate binding proteins are deployed in secondary TAXI transporters

Published:2023-03-15 Issue:7 Volume:404 Page:715-725
ISSN:1431-6730
Container-title:Biological Chemistry
language:en
Short-container-title:

Author:

Roden Anja¹^ORCID,Engelin Melanie K.¹^ORCID,Pos Klaas M.¹^ORCID,Geertsma Eric R.¹²^ORCID

Affiliation:

1. Institute of Biochemistry, Biocenter, Goethe University Frankfurt , Max-von-Laue-Strasse 9, D-60438 Frankfurt am Main , Germany

2. Max Planck Institute of Molecular Cell Biology and Genetics , Pfotenhauerstrasse 108, D-01307 Dresden , Germany

Abstract

Abstract Substrate-binding proteins (SBPs) are part of solute transport systems and serve to increase substrate affinity and uptake rates. In contrast to primary transport systems, the mechanism of SBP-dependent secondary transport is not well understood. Functional studies have thus far focused on Na+-coupled Tripartite ATP-independent periplasmic (TRAP) transporters for sialic acid. Herein, we report the in vitro functional characterization of TAXIPm-PQM from the human pathogen Proteus mirabilis. TAXIPm-PQM belongs to a TRAP-subfamily using a different type of SBP, designated TRAP-associated extracytoplasmic immunogenic (TAXI) protein. TAXIPm-PQM catalyzes proton-dependent α-ketoglutarate symport and its SBP is an essential component of the transport mechanism. Importantly, TAXIPm-PQM represents the first functionally characterized SBP-dependent secondary transporter that does not rely on a soluble SBP, but uses a membrane-anchored SBP instead.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Walter de Gruyter GmbH

Subject

Clinical Biochemistry,Molecular Biology,Biochemistry

Link

https://www.degruyter.com/document/doi/10.1515/hsz-2022-0337/pdf

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