Oleaster oil (Olea europaea sylvestris) effects on the efficiency of the reproductive system of diet-induced obese male Wistar rats
Author:
Hamenni Kahina1, Chenni Fatima Zohra1, Benalia Abdelkrim2, Djebbar Abdelhammid2, Merad Yassine3, Rebbah Kheira1, Meziani Samira1, Belhorma Abderrahmane1, Aberkane Khadidja1, Mekkiou Zohra1, Mason Onyinye Ezeh4
Affiliation:
1. Department of Biology , University of Djilali Liabes of Sidi Bel Abbes, Faculty of Natural and Life Sciences, Biotoxicology laboratory , Sidi Bel Abbes , Algeria 2. Environments and Health Research Laboratory , University of Djilali Liabes of Sidi Bel Abbes , Sidi Bel Abbes , Algeria 3. Faculty of Medicine, Central Laboratory of Hassani Abdelkader Hospital , University of Djilali Liabes of Sidi Bel Abbes , Sidi Bel Abbes , Algeria 4. Cardiff Metropolitan University, LIandaff Campus , Cardiff , UK
Abstract
Abstract
Objectives
Obesity is the result of oxidative stress (OS), which appears to be linked to male infertility. Oleaster oil has multiple therapeutic virtues due to its richness in natural bioactive compounds. This study aimed to evaluate the effect of Algerian oleaster oil on the reproductive system effectiveness of diet-induced obese male Wistar rats.
Methods
The oil was extracted with an oleodoseur from the oleaster fruits. Rats were fed for 28 weeks two diets: a normal diet (NC group, n=8) and a high-fat, high-sucrose diet (HFHS group, n=32). The obese rats in the HFHS group were then split into three groups at random: the HFHS group, which continued to receive the HFHS diet; the HFHS L-OO group; and the HFHS H-OO group, which received for 6 weeks, respectively, in combination with the HFHS diet, oleaster oil at 1.5 and 3 mL/100 g body weight (BW). Then, the animals were sacrificed. Semen and testes were removed for semen analysis, OS, and histopathological evaluation.
Results
The oleaster oil diet induces an improvement in sperm count and viability and a decrease in the percentage of fragmented sperm DNA compared to the group fed the HFHS diet, preserving the testes against OS with a highly significant decrease in testicular MDA. This beneficial effect of oleaster oil on the reproductive efficiency of rats fed the HFHS diet was dose-dependent.
Conclusions
The results showed that the oil decreased body weight, prevented OS and improved male reproductive indicators in HFHS-fed animals. The potential positive effects of oleaster oil on fertility biomarkers may be attributed to its ability to mitigate OS.
Publisher
Walter de Gruyter GmbH
Subject
Complementary and alternative medicine
Reference48 articles.
1. Jungwirth, A, Diemer, T, Dohle, G, Giwercman, A, Kopa, Z, Tournaye, H, et al.. EAU guidelines on male infertility. Eur Urol 2013;7:226–41. 2. Cooper, TG, Handelsman, DJ. Falling sperm counts and global oestrogenic pollution: postscript. Asian J Androl 2013;15:208. https://doi.org/10.1038/aja.2013.17. 3. Dupont, C, Faure, C, Sermondade, N, Boubaya, M, Eustache, F, Clément, P, et al.. Obesity leads to higher risk of sperm DNA damage in infertile patients. Asian J Androl 2013;15:622–5. https://doi.org/10.1038/aja.2013.65. 4. Jensen, TK, Heitmann, BL, Jensen, MB, Halldorsson, TI, Andersson, A-M, Skakkebæk, NE, et al.. High dietary intake of saturated fat is associated with reduced semen quality among 701 young Danish men from the general population. Am J Clin Nutr 2013;97:411–18. https://doi.org/10.3945/ajcn.112.042432. 5. Katib, A. Mechanisms linking obesity to male infertility. Cent European J Urol 2015;68:79. https://doi.org/10.5173/ceju.2015.01.435.
|
|