Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages

Author:

Gahramanova Malahat12,Ostapchuk Andriy3,Molozhava Olga2,Svyatetska Vitalina2,Rudyk Mariia2ORCID,Hurmach Yevheniia4,Gorbach Oleksandr5,Skivka Larysa2

Affiliation:

1. Nargiz Medical Center , Baku , Azerbaijan

2. ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv , Kyiv , Ukraine

3. Odessa I.I.Mechnikov National University , Odessa , Ukraine

4. Bogomolets National Medical University , Kyiv , Ukraine

5. Research Laboratory of Experimental Oncology, National Cancer Institute , Kyiv , Ukraine

Abstract

Abstract Objectives A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea. To explore the effect of polyherbal composition on the metabolism of LPS-stimulated macrophages in vitro. Methods The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC–MS). Nitric oxide (NO) assay was determined using the Griess reaction. Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry. Arginase activity was examined by colorimetric method. Results The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release. Conclusions Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.

Publisher

Walter de Gruyter GmbH

Subject

Complementary and alternative medicine

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